Addgene: How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration.

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Ailong Ke
Xiao et al

How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration.
Xiao Y, Ng S, Nam KH, Ke A
Nature. 2017 Oct 5;550(7674):137-141. doi: 10.1038/nature24020. Epub 2017 Sep 4. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
112793	His6-SUMO-pET28a-EfaCas1	To express enterococcus faecalis Cas1 protein in E. coli
112794	His6-Twin-Strep-SUMO-pET28a-EfaCas2	To express enterococcus faecalis Cas2 protein in E. coli

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