A FRET-based biosensor for measuring Galpha13 activation in single cells.
Mastop M, Reinhard NR, Zuconelli CR, Terwey F, Gadella TWJ Jr, van Unen J, Adjobo-Hermans MJW, Goedhart J
PLoS One. 2018 Mar 5;13(3):e0193705. doi: 10.1371/journal.pone.0193705. eCollection 2018. PubMed Article
Mastop M, Reinhard NR, Zuconelli CR, Terwey F, Gadella TWJ Jr, van Unen J, Adjobo-Hermans MJW, Goedhart J
PLoS One. 2018 Mar 5;13(3):e0193705. doi: 10.1371/journal.pone.0193705. eCollection 2018. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112930 | G13 sensor (Ga13_2) | A FRET sensor for G13 activation, encoded on a single plasmid. |
| 112931 | mVenus-P115-C1 | p115RhoGEF tagged with mVenus for detection of Ga13 activity |
| 112932 | LPA2R-P2A-mCherry | Plasmid co-producing the LPA2 receptor and a mCherry |
| 112933 | Ga13_2-mTq2 | Human Galpha13 tagged with mTurquoise, inserted at residue 128 |
| 112934 | At1R-P2A-mCherry | Plasmid co-producing the AT1R receptor and a mCherry |