A markerless deletion method for genetic manipulation of Burkholderia cenocepacia and other multidrug-resistant gram-negative bacteria.
Aubert DF, Hamad MA, Valvano MA
Methods Mol Biol. 2014;1197:311-27. doi: 10.1007/978-1-4939-1261-2_18. PubMed Article
Aubert DF, Hamad MA, Valvano MA
Methods Mol Biol. 2014;1197:311-27. doi: 10.1007/978-1-4939-1261-2_18. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
113635 | pDAI-SceI-SacB | Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB |