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(Plasmid #113635)


Item Catalog # Description Quantity Price (USD)
Plasmid 113635 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Mutation
    Please see Depositor Comments
  • Promoter sacB promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer GCCTTCT TGACGAGTTCTTCTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found K43E and T318P within the SacB translation compared to the best match NCBI reference sequence (ACF93714.1). The depositing laboratory confirms that these mutations should not adversely affect plasmid function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDAI-SceI-SacB was a gift from Miguel Valvano (Addgene plasmid # 113635 ; ; RRID:Addgene_113635)
  • For your References section:

    A markerless deletion method for genetic manipulation of Burkholderia cenocepacia and other multidrug-resistant gram-negative bacteria. Aubert DF, Hamad MA, Valvano MA. Methods Mol Biol. 2014;1197:311-27. doi: 10.1007/978-1-4939-1261-2_18. 10.1007/978-1-4939-1261-2_18 PubMed 25172289