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pGPI-SceI
(Plasmid #32060)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32060 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGPI-oriR6K
  • Backbone manufacturer
    Valvano
  • Vector type
    mutagenesis plasmid
  • Selectable markers
    trimethoprim

Growth in Bacteria

  • Bacterial Resistance(s)
    Trimethoprim
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1 ; any strain containing lambda pir, Pir1
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer TAACGGTTGTGGACAACAAGCCAGGG
  • 3′ sequencing primer GCCCTACACAAATTGGGAGATATATC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGPI-SceI was a gift from Miguel Valvano (Addgene plasmid # 32060 ; http://n2t.net/addgene:32060 ; RRID:Addgene_32060)
  • For your References section:

    A system for the construction of targeted unmarked gene deletions in the genus Burkholderia. Flannagan RS, Linn T, Valvano MA. Environ Microbiol. 2008 Jun;10(6):1652-60. Epub 2008 Mar 13. 10.1111/j.1462-2920.2008.01576.x PubMed 18341581