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lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2
(Plasmid #170378)


Item Catalog # Description Quantity Price (USD)
Plasmid 170378 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    lentiCRISPR v2
  • Backbone manufacturer
    Feng Zhang Addgene plasmid #52961
  • Backbone size w/o insert (bp) 7546
  • Total vector size (bp) 13818
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
  • Species
  • Insert Size (bp)
  • Promoter human Synapsin promoter
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    dCas9-KRAB-MeCP2 was a gift from Alejandro Chavez & George Church (Addgene plasmid # 110821 ; ; RRID:Addgene_110821)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2 was a gift from Jeremy Day (Addgene plasmid # 170378 ; ; RRID:Addgene_170378)
  • For your References section:

    A Cre-dependent CRISPR/dCas9 system for gene expression regulation in neurons. Carullo NVN, Hinds JE, Revanna JS, Tuscher JJ, Bauman AJ, Day JJ. eNeuro. 2021 Jul 26. pii: ENEURO.0188-21.2021. doi: 10.1523/ENEURO.0188-21.2021. 10.1523/ENEURO.0188-21.2021 PubMed 34321217