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A Cre-dependent CRISPR/dCas9 system for gene expression regulation in neurons.
Carullo NVN, Hinds JE, Revanna JS, Tuscher JJ, Bauman AJ, Day JJ
eNeuro. 2021 Jul 26. pii: ENEURO.0188-21.2021. doi: 10.1523/ENEURO.0188-21.2021.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
164575lenti SYN-SVI-dCas9-VPRExpresses an intron-containing dCas9-VPR fusion driven by human SYN promoter
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164576lenti SYN-SVI-DIO-dCas9-VPRExpresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
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170378lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
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