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lenti SYN-SVI-dCas9-VPR
(Plasmid #164575)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 164575 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    lentiCRISPR v2
  • Backbone manufacturer
    Feng Zhang Addgene plasmid #52961
  • Backbone size w/o insert (bp) 7546
  • Total vector size (bp) 13968
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FLAG-dCas9-VPR containing an SV40 intron
  • Species
    Synthetic
  • Insert Size (bp)
    6422
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti SYN-SVI-dCas9-VPR was a gift from Jeremy Day (Addgene plasmid # 164575 ; http://n2t.net/addgene:164575 ; RRID:Addgene_164575)
  • For your References section:

    A Cre-dependent CRISPR/dCas9 system for gene expression regulation in neurons. Carullo NVN, Hinds JE, Revanna JS, Tuscher JJ, Bauman AJ, Day JJ. eNeuro. 2021 Jul 26. pii: ENEURO.0188-21.2021. doi: 10.1523/ENEURO.0188-21.2021. 10.1523/ENEURO.0188-21.2021 PubMed 34321217