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Synthetic Genome Defenses against Selfish DNA Elements Stabilize Engineered Bacteria against Evolutionary Failure.
Geng P, Leonard SP, Mishler DM, Barrick JE
ACS Synth Biol. 2019 Mar 15;8(3):521-531. doi: 10.1021/acssynbio.8b00426. Epub 2019 Feb 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113646pDCAF-ADemethylates methylxanthines.
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113647pDCAF-BDemethylates methylxanthines.
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113648pDCAF-CDemethylates methylxanthines.
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113649pDCAF-ABDemethylates methylxanthines.
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113650pDCAF-ACDemethylates methylxanthines.
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113651pDCAF-BCDemethylates methylxanthines.
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113652pDCAF-ABCDemethylates methylxanthines.
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113653delta guaBBase strain that the pDCAF plasmids (Addgene #113646-113652) must be in.
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113654pJ23119-sgRNAsgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.
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113655T5-sgRNAsgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.
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113656Native promoter dCas9dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.
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113657TDK promoter dCas9dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.
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