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A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B.
Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, Vleugel M, van Osch MH, Snel B, Perrakis A, Kops GJ
J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. Epub 2013 Apr 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
114044pCDNA5-LAP-MPS1-WTExpression of exogenous LAP tagged MPS1 WT
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114045pCDNA5-LAP-MPS1-KDExpression of exogenous LAP tagged MPS1 Kinase-dead
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114046pCDNA5-LAP-MPS1-d60Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)
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114047pCDNA5-LAP-MPS1-d200Expression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted)
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114048pCDNA5-LAP-MPS1-dTPRExpression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)
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114049pLacI-GFP-HEC1 WTExpression of exogenous LacI-GFP-HEC1 WT
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114050pLacI-GFP-HEC1-d207Expression of exogenous LacI-GFP-HEC1 delta 207
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114051pcDNA5-hygro-eGFP-HEC1-d207Expression of exogenous eGFP HEC1 delta 207
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Antibodies from Article