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pLacI-GFP-HEC1 WT
(Plasmid #114049)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 114049 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-HEC1
  • Species
    H. sapiens (human)
  • Mutation
    WT HEC1 sequence
  • Entrez Gene
    NDC80 (a.k.a. HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLacI-GFP-HEC1 WT was a gift from Geert Kops (Addgene plasmid # 114049 ; http://n2t.net/addgene:114049 ; RRID:Addgene_114049)
  • For your References section:

    A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B. Nijenhuis W, von Castelmur E, Littler D, De Marco V, Tromer E, Vleugel M, van Osch MH, Snel B, Perrakis A, Kops GJ. J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. Epub 2013 Apr 8. 10.1083/jcb.201210033 PubMed 23569217