Addgene: Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon.

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Mingshan Xue
Messier et al

Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon.
Messier JE, Chen H, Cai ZL, Xue M
Elife. 2018 Aug 9;7. pii: 38506. doi: 10.7554/eLife.38506. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
114367	pCAG-hChR2(H134R)-EYFP	expresses channelrhodopsin 2 fused with EYFP
114368	pAAV-EF1α-DIO-ReaChR-P2A-dTomato	expresses a Cre-dependent red light-shifted channelrhodopsin variant
114369	pAAV-EF1α-FRT-FLEX-GtACR2-EYFP	expresses Flpo recombinase-dependent GtACR2
114370	pAAV-EF1α-FRT-FLEX-GtACR1-EYFP	expresses Flpo recombinase-dependent GtACR1
114371	pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD	expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment
114372	pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C	expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment
114373	pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL	expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment
114374	pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC	expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment
114375	pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C	expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment
114376	pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC	expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment
114377	pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC	expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting
114378	pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP	expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

Antibodies from Article