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Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon.
Messier JE, Chen H, Cai ZL, Xue M
Elife. 2018 Aug 9;7. pii: 38506. doi: 10.7554/eLife.38506.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
114367pCAG-hChR2(H134R)-EYFPexpresses channelrhodopsin 2 fused with EYFP
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114368pAAV-EF1α-DIO-ReaChR-P2A-dTomatoexpresses a Cre-dependent red light-shifted channelrhodopsin variant
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114369pAAV-EF1α-FRT-FLEX-GtACR2-EYFPexpresses Flpo recombinase-dependent GtACR2
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114370pAAV-EF1α-FRT-FLEX-GtACR1-EYFPexpresses Flpo recombinase-dependent GtACR1
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114371pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBDexpresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment
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114372pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1Cexpresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment
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114373pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LLexpresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment
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114374pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnCexpresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment
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114375pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1Cexpresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment
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114376pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnCexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment
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114377pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnCexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting
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114378pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFPexpresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment
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