Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors.
Iyer S, Mir A, Vega-Badillo J, Roscoe BP, Ibraheim R, Zhu LJ, Lee J, Liu P, Luk K, Mintzer E, Guo D, Soares de Brito J, Emerson CP Jr, Zamore PD, Sontheimer EJ, Wolfe SA
CRISPR J. 2022 Oct;5(5):685-701. doi: 10.1089/crispr.2022.0058. Epub 2022 Sep 7. PubMed Article
Iyer S, Mir A, Vega-Badillo J, Roscoe BP, Ibraheim R, Zhu LJ, Lee J, Liu P, Luk K, Mintzer E, Guo D, Soares de Brito J, Emerson CP Jr, Zamore PD, Sontheimer EJ, Wolfe SA
CRISPR J. 2022 Oct;5(5):685-701. doi: 10.1089/crispr.2022.0058. Epub 2022 Sep 7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 115909 | pBS-f1(+)-ACTB-mEGFP | donor to insert EGFP at the ACTB locus in human cells |
| 115910 | pBS-f1(+)-SEC61B-mEGFP | donor to insert EGFP at the SEC61B locus in human cells |
| 115911 | pBS-f1(+)-TOMM20-mEGFP | donor to insert EGFP at the TOMM20 locus in human cells |
| 115912 | pBS-f1(+)-GAPDH-IRES-dTomato | donor to insert IRES-dTomato at the GAPDH locus in human cells |
| 115913 | pBS-f1(+)-SEC61B-dTomato | donor to insert dTomato at the SEC61B locus in human cells |
| 115914 | pBS-f1(+)-TOMM20-dTomato | donor to insert dTomato at the TOMM20 locus in human cells |
| 115915 | pBS-f1(+)-ACTB-iTagRFP-T | donor to insert iTagRFP-T at the ACTB locus in human cells |
| 115916 | pBS-f1(-)-ACTB-iTagRFP-T | donor to insert iTagRFP-T at the ACTB locus in human cells |
| 115917 | pBS-f1(-)-ACTB-mEGFP | donor to insert EGFP at the ACTB locus in human cells |
| 115918 | pBS-f1(-)-GAPDH-IRES-EGFP | donor to insert IRES-EGFP at the GAPDH locus in human cells |
| 115919 | pBS-f1(-)-GAPDH-IRES-dTomato | donor to insert IRES-dTomato at the GAPDH locus in human cells |
| 115920 | pBS-f1(-)-SEC61B-dTomato | donor to insert dTomato at the SEC61B locus in human cells |
| 115921 | pBS-f1(-)-SEC61B-mEGFP | donor to insert mEGFP at the SEC61B locus in human cells |
| 115922 | pBS-f1(-)-TOMM20-dTomato | donor to insert dTomato at the TOMM20 locus in human cells |
| 115923 | pBS-f1(-)-TOMM20-mEGFP | donor to insert EGFP at the TOMM20 locus in human cells |
| 117111 | pCVL-UCOE-SFFV-TLR-MCV1 | Lentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1. |
| 117405 | pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1 | SpyCas9 sgRNA 1 targeting TLR2.0 |
| 117406 | pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2 | SpyCas9 sgRNA 2 targeting TLR2.0 |
| 117407 | pLKO.1-puro-U6-NmeCas9-TLR-MCV1-sgRNA | NmeCas9 sgRNA targeting TLR2.0 |
| 117408 | pLKO.1-puro-U6-CjeCas9-TLR-MCV1-sgRNA | CjeCas9 sgRNA targeting TLR2.0 |
| 117409 | pLKO.1-puro-U6-SauCas9-TLR-MCV1-sgRNA | SauCas9 sgRNA targeting TLR2.0 |
| 117410 | pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNA | LbCas12a-gRNA targeting TLR2.0 |
| 117411 | pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA | AsCas12a-gRNA targeting TLR2.0 |
| 117412 | U6-FnCas12a-TLR-MCV1-gRNA | FnCas12a-gRNA targeting TLR2.0 |