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An inducible genome editing system for plants.
Wang X, Ye L, Lyu M, Ursache R, Loytynoja A, Mahonen AP
Nat Plants. 2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 10.1038/s41477-020-0695-2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
118385p221z-CAS9p-t35sEntry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system
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118386p221z-CAS9p-TagRFP-t35sEntry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system
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118387p221z-dCAS9p-t35sEntry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system
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118388p221z-AtMIR390aEntry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.
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118389p2R3z-BASI-ccdB-BSAIEntry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system
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118390p2R3z-AtU3b-tRNA-ccdB-gRNA Entry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway system
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133747pFG7m34GWThree-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana
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133748pFRm43GWThree-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thaliana
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