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Addgene
Phase separation-deficient TDP43 remains functional in splicing.
Schmidt HB, Barreau A, Rohatgi R
Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
107799pHBS931 TDP43 RRM-GFP F-STo test the effect of sequence on TDP43 droplet properties
107800pHBS935 TDP43 RRM-GFP M-STo test the effect of sequence on TDP43 droplet properties
107801pHBS934 TDP43 RRM-GFP M-VTo test the effect of sequence on TDP43 droplet properties
107804pHBS1037 TDP43 RRM-GFP 6xΦTo test the effect of sequence on TDP43 droplet properties
107805pHBS1038 TDP43 RRM-GFP 4xΦTo test the effect of sequence on TDP43 droplet properties
107806pHBS1039 TDP43 RRM-GFP 2xΦTo test the effect of sequence on TDP43 droplet properties
107807pHBS1256 TDP43 RRM-GFP W385GTo test the effect of sequence on TDP43 droplet properties
107822pHBS966 TDP43 RRM-GFP G309FTo test the effect of sequence on TDP43 droplet properties
107824pHBS968 TDP43 RRM-GFP G309STo test the effect of sequence on TDP43 droplet properties
107830pHBS1280 TDP43 RRM-GFP G348FTo test the effect of sequence on TDP43 droplet properties
107831pHBS1279 TDP43 RRM-GFP G348VTo test the effect of sequence on TDP43 droplet properties
107837pHBS1291 TDP43 RRM-GFP G368WTo test the effect of sequence on TDP43 droplet properties
107838pHBS1292 TDP43 RRM-GFP G368W+W385GTo test the effect of sequence on TDP43 droplet properties
107856pHBS1052 TDP43 RRM-GFP R-KTo test the effect of sequence on TDP43 droplet properties
107857pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9To knock-out TDP43
107858pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9To knock-out TDP43
118791pHBS1383 TDP43 RRM-GFP FYW-STo test the effect of sequence on TDP43 RRM-GFP droplet properties
118792pHBS1385 TDP43 RRM-GFP VLIM-STo test the effect of sequence on TDP43 RRM-GFP droplet properties
118793pHBS1384 TDP43 RRM-GFP FYW-LTo test the effect of sequence on TDP43 RRM-GFP droplet properties
118794pHBS1386 TDP43 RRM-GFP VLIM-FTo test the effect of sequence on TDP43 RRM-GFP droplet properties
118795pHBS1387 TDP43 RRM-GFP KRED-STo test the effect of sequence on TDP43 RRM-GFP droplet properties
118796pHBS1388 TDP43 RRM-GFP K-RTo test the effect of sequence on TDP43 RRM-GFP droplet properties
118797pHBS1147 GFP-TDP43To test the effect of sequence on nuclear body formation and splicing of full-length TDP43
118798pHBS1084 GFP-TDP43 deltaCTDTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43
118799pHBS1410 GFP-TDP43 F-STo test the effect of sequence on nuclear body formation and splicing of full-length TDP43
118800pHBS1411 GFP-TDP43 FYW-STo test the effect of sequence on nuclear body formation and splicing of full-length TDP43
118801pHBS1412 GFP-TDP43 FYW-LTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43
118802pHBS1413 GFP-TDP43 VLIM-FTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43
118803pHBS1389 IBB-GFP-mCherry3EFluorescence-based TDP43-dependent splicing reporter
118804pHBS1396 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]To test the effect of sequence on TDP43 splicing activity
118805pHBS1393 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaRRM]To test the effect of sequence on TDP43 splicing activity
118806pHBS1394 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaCTD]To test the effect of sequence on TDP43 splicing activity
118807pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]To test the effect of sequence on TDP43 splicing activity
118808pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]To test the effect of sequence on TDP43 splicing activity
118809pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S]To test the effect of sequence on TDP43 splicing activity
118810pHBS1405 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-F]To test the effect of sequence on TDP43 splicing activity
118811pHBS1398 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W]To test the effect of sequence on TDP43 splicing activity
118812pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]To test the effect of sequence on TDP43 splicing activity
118813pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W]To test the effect of sequence on TDP43 splicing activity
118814pHBS1400 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295F]To test the effect of sequence on TDP43 splicing activity
121992pHBS941 TDP43 RRM-GFP VLIMFYW-STo test the effect of sequence on TDP43 RRM-GFP droplet properties
133320pHBS834 H14-SUMO-TDP43 WT-TEV-mCherryBacterial expression of TDP43 with WT CTD
133321pHBS1551 H14-SUMO-TDP43 F-S-TEV-mCherryBacterial expression of TDP43 with F-S mutant CTD
133322pHBS1552 H14-SUMO-TDP43 FYW-S-TEV-mCherryBacterial expression of TDP43 with FYW-S mutant CTD
133323pHBS1415 GFP-TDP43 VLIM-STo test the effect of sequence on nuclear body formation and splicing of full-length TDP43
133324pHBS1500 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaNTD]To test the effect of sequence on TDP43 splicing activity
133325pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]To test the effect of sequence on TDP43 splicing activity
133326pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD]To test the effect of sequence on TDP43 splicing activity
133327pHBS1503 [IBB-GFP-mCherry3E]-[BFP-TDP43 WT]To test the effect of sequence on TDP43 splicing activity
133328pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]To test the effect of sequence on TDP43 splicing activity
133329pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S]To test the effect of sequence on TDP43 splicing activity
133330pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]To test the effect of sequence on TDP43 splicing activity
133331pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S]To test the effect of sequence on TDP43 splicing activity
133332pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]To test the effect of sequence on TDP43 splicing activity
133333pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]To test the effect of sequence on TDP43 splicing activity

Antibodies from Article