A Forster resonance energy transfer sensor for live-cell imaging of mitogen-activated protein kinase activity in Arabidopsis.
Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, Zhang S, Krysan PJ
Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164. PubMed Article
Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, Zhang S, Krysan PJ
Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118935 | pET-SOMA | Used to express the SOMA FRET sensor in E. coli. |
| 118936 | pET-SOMA-T679A | Used to express the T679A mutant form of the SOMA FRET sensor in E. coli. |
| 118937 | pSOMA-NLS | Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana |
| 118938 | pSOMA-T679A-NLS | Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana |
| 118939 | pSOMA-NES | Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana |
| 118940 | pSOMA-T679A-NES | Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana |