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Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning.
Guardia CM, De Pace R, Sen A, Saric A, Jarnik M, Kolin DA, Kunwar A, Bonifacino JS
PLoS Biol. 2019 May 17;17(5):e3000279. doi: 10.1371/journal.pbio.3000279. eCollection 2019 May.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120163mCh-KIF5A*-strepExpresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin
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120164mCh-KIF5B*-strepExpresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin
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120165mCh-KIF1A*-strepExpresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin
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120166mCh-KIF13A*-strepExpresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin
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120167mCh-KIF17*-strepExpresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin
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120168mCh-BICD2*-strepExpresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin
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120169Strep-KIFC1*-mChExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)
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120170HA-KIF5B*-strepExpresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin
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120171Strep-KIFC1*-HAExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag
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120172LAMP1-SBP-GFPExpresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)
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120173TOM20*-SBP-GFPExpresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)
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120174PEX3*-SBP-GFPExpresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag)
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120175GFP-SBP-VAP-A*Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A
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Antibodies from Article