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Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya H, Moore BS
Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 10.1016/j.chembiol.2019.02.004.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120229pCAP-BACTAR cloning in yeast and low-copy maintenance of large constructs in E. coli
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120230pJZ001Contains intB13 integrase casette for knock-in to pCAP-BAC backbone
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120231pJZ002CRISPR-cas9 targeting in E. coli with ampicillin resistance
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120232pCAP03-sp44/p21Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.
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120233pCAP03-act1pCarries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.
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120234pCAP03-ermE*pCarries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.
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