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Expanding the potential of CRISPR-Cpf1 based genome editing technology in the cyanobacterium Anabaena PCC 7120.
Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC
ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
122185pCpf1Bacterial genome editing plasmid with kanamycin resistance marker
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122186pCpf1-spBacterial genome editing plasmid with spectinomycin resistance marker
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122187pCpf1bBacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker
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122188pCpf1b-spBacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker
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