Purpose(Empty Backbone) Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122188||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Copy numberLow Copy
- Cloning method Ligation Independent Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCpf1b-sp was a gift from Cheng-Cai Zhang (Addgene plasmid # 122188 ; http://n2t.net/addgene:122188 ; RRID:Addgene_122188)
For your References section:Expanding the potential of CRISPR-Cpf1 based genome editing technology in the cyanobacterium Anabaena PCC 7120. Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC. ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437. 10.1021/acssynbio.8b00437 PubMed 30525474