A toolkit for DNA assembly, genome engineering and multicolor imaging for C. elegans.
Sands B, Burnaevskiy N, Yun SR, Crane MM, Kaeberlein M, Mendenhall A
Transl Med Aging. 2018 Jan;2:1-10. doi: 10.1016/j.tma.2018.01.001. Epub 2018 Apr 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110915 | BSP179 | For cloning expression cassettes by HR in yeast, Universal MosSCI compatible |
| 110917 | BSP188 | For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned |
| 122249 | BSP602 | expression vector for mScarlet under EFT3 Promoter |
| 122250 | BSP605 | expression vector for mCerulean under EFT3 promoter |
| 122251 | BSP606 | expression vector for mECitrine under EFT3 promoter |
| 122252 | BSP607 | expression vector for mKO2 under EFT3 promoter |
| 122253 | BSP608 | expression vector for mNeptune under EFT3 promoter |
| 122254 | BSP609 | expression vector for mTFP under EFT3 promoter |
| 122255 | BSP610 | expression vector for mtagBFP2 under EFT3 promoter |
| 122256 | BSP611 | expression vector for mtagRFP-T under EFT3 promoter |