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A CRISPR Interference Platform for Efficient Genetic Repression in Candida albicans.
Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS
mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 10.1128/mSphere.00002-19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
122377pRS143CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
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122378pRS159CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
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122379pRS263CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon
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122380pRS267CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon
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