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Single-color, ratiometric biosensors for detecting signaling activities in live cells.
Ross BL, Tenner B, Markwardt ML, Zviman A, Shi G, Kerr JP, Snell NE, McFarland JJ, Mauban JR, Ward CW, Rizzo MA, Zhang J
Elife. 2018 Jul 3;7. pii: 35458. doi: 10.7554/eLife.35458.
PubMed

Plasmids from Article

ID Plasmid Purpose  
123329pcDNA3-Venus-cpVenus-FLARE-AKARYellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
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123330pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.
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123331pcDNA3-Venus-Venus-FLARE-AKARYellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
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123332pcDNA3-GFP-GFP-FLARE-AKARGreen-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
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123333pcDNA3-mCherry-mCherry-FLARE-AKARRed-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
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123335pcDNA3-Cerulean3-Cerulean3-FLARE-AKARCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
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123336pcDNA3-Cerulean3-cpCerulean3-FLARE-AKARCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.
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123337pcDNA3-Lyn-Venus-cpVenus-FLARE-AKARPlasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
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123338pcDNA3-Dakap-Venus-cpVenus-FLARE-AKARMitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
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123339pcDNA3-Venus-cpVenus-FLARE-EKAR-EVYellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
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123340pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.
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123341pcDNA3-mCherry-mCherry-FLARE-EKAR-EVRed-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.
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123342pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EVCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.
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123343pcDNA3-Venus-cpVenus-FLARE-CKARYellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
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123344pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.
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123345pcDNA3-mCherry-mCherry-FLARE-CKARRed-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.
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123346pcDNA3-Cerulean3-Cerulean3-FLARE-CKARCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.
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123348pcDNA3-CKAR2Improved FRET-based biosensor for monitoring Protein Kinase C activity.
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123350pcDNA3-Venus-cpVenus-FLARE-CameleonYellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
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123351pcDNA3-Venus-Venus-FLARE-CameleonYellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
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123352pcDNA3-mCherry-mCherry-FLARE-CameleonRed-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
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123353pcDNA3-Cerulean3-Cerulean3-FLARE-CameleonCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
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123354pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ERCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.
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123355pcDNA3-Venus-cpVenus-FLARE-ICUEYellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
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