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Rewiring bacterial two-component systems by modular DNA-binding domain swapping.
Schmidl SR, Ekness F, Sofjan K, Daeffler KN, Brink KR, Landry BP, Gerhardt KP, Dyulgyarov N, Sheth RU, Tabor JJ
Nat Chem Biol. 2019 Jul;15(7):690-698. doi: 10.1038/s41589-019-0286-6. Epub 2019 May 20. PubMed Article

Plasmids from Article

ID Plasmid Purpose
124712pSR305Expression of uhpB under Ptac
124713pSR348Expression of narX under Ptac
124714pKD251Expression of torS under Ptac and torT under PD59_luxR
125069pKD276Expression of SO_0621 under Ptac
125070pKD278Expression of SO_2192 under Ptac
125071pKD280Expression of SO_4387 under Ptac
125072pKD282Expression of SO_4427 under Ptac
125073pKD284Expression of SO_4488 under Ptac
125074pKD286Expression of SO_4622 under Ptac
125075pKD288Expression of SO_4638 under Ptac
125076pSR62Expression of ompR under PLtetO-1, output promoter PompF146
125077pKD250.2Expression of torR under PLtetO-1, output promoter PtorCAD129
125078pSR353Expression of narL under PLtetO-1, output promoter PdcuS77
125079pSR300.3Expression of uhpA under PLtetO-1, output Promoter PuhpT99
125080pSR40.13Expression of ompR(1-118aa)-ccaR(115-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125081pSR40.14Expression of ompR(1-122aa)-ccaR(119-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125082pSR40.15Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125083pSR40.16Expression of ompR(1-140aa)-ccaR(130-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125084pSR40.18Expression of ompR(1-150aa)-ccaR(140-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125085pSR40.25Expression of ompR(1-110aa)-ccaR(107-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125086pSR40.26Expression of ompR(1-113aa)-ccaR(110-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125087pSR40.28Expression of ompR(1-138aa)-ccaR(128-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125088pSR40.29Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125089pSR40.30Expression of ompR(1-142aa)-ccaR(132-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172
125090pSR240Expression of ompR(1-137aa)-psdR(134-237aa) chimera under PLtetO-1, output Promoter PpsdA110
125091pSR241Expression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181
125092pSR276.4Expression of ompR(1-122aa)-psdR(119-237aa) chimera under PLtetO-1, output Promoter PpsdA110
125093pSR284Expression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143
125094pKD263Expression of torR(1-134aa)-ccaR(127-234aa), ompR137 chimera under PLtetO-1, output Promoter PcpcG2-172
125095pKD265Expression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
125096pKD266Expression of torR(1-134aa)-yxdJ(132-229aa), ompR137 chimera under PLtetO-1, output Promoter PyxdJ181
125097pKD275Expression of SO_0622(1-129aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
125098pKD277Expression of SO_2193(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
125099pKD279Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
125100pKD281Expression of SO_4428(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
125101pKD283.2Expression of SO_4487(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
125102pKD285Expression of SO_4623(1-124aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
125103pKD287Expression of SO_4637(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
125104pSR357.6Expression of narL(1-126aa)-ydfI(121-213aa) chimera under PLtetO-1, output Promoter PydfJ115
125105pSR357.9Expression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115
125106pSR357.10Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115
125107pSR357.12Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115
125108pSR357.20Expression of narL(1-154aa)-ydfI(148-213aa) chimera under PLtetO-1, output Promoter PydfJ115
125109pSR357.24Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115
125110pSR357.27Expression of narL(1-120aa)-ydfI(115-213aa) chimera under PLtetO-1, output Promoter PydfJ115
125111pSR357.28Expression of narL(1-122aa)-ydfI(117-213aa) chimera under PLtetO-1, output Promoter PydfJ115
125112pSR358Expression of narL(1-131aa)-liaR(127-211aa) chimera under PLtetO-1, output Promoter PliaI121
125113pSR358.2Expression of narL(1-126aa)-liaR(122-211aa) chimera under PLtetO-1, output Promoter PliaI121
125114pSR358.4Expression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121
125115pSR359.4Expression of narL(1-159aa)-uhpA(142-129aa) chimera under PLtetO-1, output Promoter PuhpT99
125116pSR322Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115
125117pSR322.2Expression of uhpA(1-118aa)-ydfI(121-213aa), narL126 chimera under PLtetO-1, output Promoter PydfJ115
125118pSR322.3Expression of uhpA(1-121aa)-ydfI(124-213aa), narL129 chimera under PLtetO-1, output Promoter PydfJ115
125119pSR322.4Expression of uhpA(1-141aa)-ydfI(153-213aa), narL159 chimera under PLtetO-1, output Promoter PydfJ115

Antibodies from Article