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A modular cloning toolkit for genome editing in plants.
Hahn F, Korolev A, Sanjurjo Loures L, Nekrasov V
BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
125760pAK-EL-01Endlinker for tRNA-gRNA system when using only one guide
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125761pAK-EL-02Endlinker for tRNA-gRNA system when using only two guides
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125762pAK-EL-03Endlinker for tRNA-gRNA system when using only three guides
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125763pAK-EL-04Endlinker for tRNA-gRNA system when using only four guides
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125764pAK-EL-05Endlinker for tRNA-gRNA system when using only five guides
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125798pFH13Level0 SpCas9, wheat codon optimized
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125799pFH14Level0 SaCas9, Arabidopsis codon optimized
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125800pFH15Level0 StCas9, Arabidopsis codon optimized
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125801pFH16Level0 FnCas12a, rice codon optimized
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125803pFH17Level0 LbCas12a, rice codon optimized
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125808pFH22Level0 xCas9 3.7
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125809pFH32Level0 SpCas9-NG, human codon optimized
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125810pFH45Level0 nSpCas9-ABE7.10 base changer, human codon optimized
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125811pFH46Level0 FnCas12a, human codon optimized
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125812pFH47Level0 LbCas12a, human codon optimized
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125813pFH48Level0 Csy4
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125814pFH55Level0 nSpCas9-PmCDA, Arabidopsis codon optimized
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125815pFH76Level0 ScCas9, wheat codon optimized
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125816pFH77Level0 enCas9-PolI3M-TBD, wheat codon optimized
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125818pFH79Level0 A3A-PBE, wheat codon optimized
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125819pFH91Level0 nScCas9 (nickase), wheat codon optimized
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125820pFH92Level0 nScCas9-ABE7.10, wheat/human codon optimized
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125879pFH40Level0 Switchgrass PvUBI1 Pol II promoter
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125880pFH41Level0 Parsley PcUBI4-2 Pol II promoter
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125881pFH42Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter
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125882pFH56Level0 Rice OsACT1 Pol II promoter
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125883pFH57Level0 Soybean GmUBI Pol II promoter
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125884pFH31Level0 Wheat TaU3 Pol III promoter
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125885pFH33Level0 Wheat TaU3 Pol III promoter with transcription Start-A
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126008pFH34Level0 Arabidopsis AtU6-26 Pol III promoter
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126009pFH35Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G
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126010pFH36Level0 Rice OsU6-2 Pol III promoter
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126011pFH37Level0 Rice OsU6-2 Pol III promoter with transcription Start-G
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126012pFH38Level0 Rice OsU3 Pol III promoter
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126013pFH39Level0 Rice OsU3 Pol III promoter with transcription Start-A
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126020pFH43Level0 Pea RbSC E9 terminator
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126021pFH44Level0 Pea 3A terminator
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128180pFH23Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt
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128181pFH52Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
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128182pFH53Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At
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128183pFH54Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
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128184pFH58Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt
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128185pFH59Level1 ZmUBIp::StCas9 (Arabidopsis codon optimized):NOSt
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128186pFH60Level1 ZmUBIp::FnCas12a (rice codon optimized):NOSt
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128187pFH61Level1 ZmUBIp::LbCas12a (rice codon optimized):NOSt
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128188pFH68Level1 ZmUBIp::SpCas9-NG (human codon optimized):NOSt
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128189pFH69Level1 ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt
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128190pFH70Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
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128191pFH80Level1 ZmUBIp::xCas9 3.7:NOSt
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128192pFH81Level1 ZmUBIp::ScCas9 (wheat codon optimized):NOSt
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128193pFH82Level1 ZmUBIp::enCas9-Poll3M-TBD (wheat codon optimized):NOSt
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128195pFH84Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt
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128196pFH93Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt
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128197pFH109Level1 ZmUBIp::nScCas9 (wheat codon optimized):NOSt
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128198pFH85classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
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128199pFH86improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
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128200pFH87sgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33)
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128201pFH88sgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
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128202pFH89crRNA backbone for LbCas12a, combine with TaU3 promoter module (pFH33)
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128203pFH90crRNA backbone for FnCas12a/CmsI nucleases, combine with TaU3 promoter module (pFH33)
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128204pFH99classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
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128205pFH100improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
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128206pFH101sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)
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128207pFH102sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
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128208pFH103crRNA backbone for LbCas12a, combine with AtU6-26 promoter module (pFH35)
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128209pFH104crRNA backbone for FnCas12a/CmsI nucleases, combine with AtU6-26 promoter module (pFH35)
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128210pFH113improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
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128211pFH49improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
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128212pFH50improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
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128213pFH51improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
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128214pFH71improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
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128215pAK002improved sgRNA backbone for tRNA-gRNA array, Position 2
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128216pAK003improved sgRNA backbone for tRNA-gRNA array, Position 3
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128217pAK004improved sgRNA backbone for tRNA-gRNA array, Position 4
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128218pAK005improved sgRNA backbone for tRNA-gRNA array, Position 5
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128219pAK006improved sgRNA backbone for tRNA-gRNA array, Position 6
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128220pAK007classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
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128221pFH72classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
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128222pFH73classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
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128223pFH74classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
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128224pFH75classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
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128225pAK008classic sgRNA backbone for tRNA-gRNA array, Position 2
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128226pAK009classic sgRNA backbone for tRNA-gRNA array, Position 3
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128227pAK010classic sgRNA backbone for tRNA-gRNA array, Position 4
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128228pAK011classic sgRNA backbone for tRNA-gRNA array, Position 5
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128229pAK012classic sgRNA backbone for tRNA-gRNA array, Position 6
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128408pFH114Level1 ZmUBIp::BAR:NOSt
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131763pFH24Level0 SpCas9, wheat codon optimized (version 2)
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131764pFH25Level0 SpCas9, wheat codon optimized (version 3)
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131765pFH66Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
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131766pFH67Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt
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136547pFH132Level1 ZmUBIp::FnCas12a (human codon optimized):NOSt
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136548pFH133Level1 ZmUBIp::LbCas12a (human codon optimized):NOSt
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136549pFH130crRNA backbone for FnCas12a/CmsI nucleases, combine with OsU6-2 promoter module (pFH37)
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136550pFH131crRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39)
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Recombinant Antibodies from Article