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De novo design of bioactive protein switches.
Langan RA, Boyken SE, Ng AH, Samson JA, Dods G, Westbrook AM, Nguyen TH, Lajoie MJ, Chen Z, Berger S, Mulligan VK, Dueber JE, Novak WRP, El-Samad H, Baker D
Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 10.1038/s41586-019-1432-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
127200pAN1578Progesterone inducible expression of KeyA-BFP
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127201pAN1579Progesterone inducible expression of KeyC-BFP
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127202pAN1572Dual inducible expression of Venus-degronSwitchA and KeyA-BFP
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127203pAN1935Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC
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127204pAN1735Dual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLS
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127205pAN1646pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS
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127206pAN1777Dual inducible expression of SynTF-mScarlet-nesSwitchA and KeyA-BFP-NLS
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127246pAN2981Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP
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