Oskar Laur Lab: Emory Custom Cloning Core Plasmids - Oskar Laur

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
128023	pCDNA3- c-terminal 6xMyc	Mammalian expression vector with C terminal 6xMyc tag
128024	pcDNA3-c-terminal mouseFc	Mammalian expression vector with C terminal mFC tag
128025	pcDNA3-C-terminal GST	Mammalian expression vector with C terminal GST tag
128026	pcDNA3-C-terminal CyPet	Mammalian expression vector with C terminal CyPet tag
128027	pcDNA3-C-terminal YPet	Mammalian expression vector with C terminal YPet tag
128028	pcDNA3-IL-2 signal	Mammalian expression vector with N terminal IL-2 signal peptide
128029	pcDNA3-IL-2 signal_CyPet	Mammalian expression vector with N terminal IL-2 signal peptide and CyPet
128030	pcDNA3-IL- signal_YPet	Mammalian expression vector with N terminal IL-2 signal peptide and YPet
128031	pcDNA3-IL-2 signal_rabbit Fc	Mammalian expression vector with N terminal IL-2 signal peptide and rabbit Fc
128032	pcDNA3-IL-2 signal_GST	Mammalian expression vector with N terminal IL-2 signal peptide and GST
128033	pcDNA3-IL-2signal-6xMyc	Mammalian expression vector with N terminal IL-2 signal peptide and 6xMyc tag
128034	pcDNA3.1-HA	Mammalian expression vector with N terminal HA tag
128035	pVL1393-6xMyc	Baculovirus expression vector with C-terminal 6xMyc tag
128036	pShuttle-CMV-IRES-GFP	Adenoviral shuttle vector with CMV promoter, coexpresses EGFP
128040	pcDNA3 DSred express2	Mammalian expression vector with N terminal DsRed express2
128042	pCDNA3 IL-2 signal mFc	Mammalian expression vector with C terminal IL-2 signal peptide and mFc
128043	pMA2780 lenti tet on EGFP	Tet inducible Lentiviral vector with EGFP co-expression
128044	pLEX-MCS PGK promoter	Lentiviral vector with PGK promoter
128045	pLEX-MCS UBC promoter	Lentiviral vector with UBC promoter
128046	pGL3basic luciferase and renilla_polyA	pGL3 basic luciferase reporter with co-expression of renilla luciferase
128047	pcDNA3.1 acGFP	Mammalian expression vector with N terminal AcGFP
128048	pLEX MCS snap tag	Lentiviral vector with snap tag
128049	pShuttle-CMV-GFP U6promoter	Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker
128050	pShuttle-CMV-GFP H1promoter	Adenoviral shuttle vector for shRNA expression from H1 promoter with GFP marker
128051	pLex MCS mCherry Myc	Lentiviral vector with mCherry Myc
128052	prs316 CUP-GFP	Yeast expression vector for N terminal GFP fusions
128053	pGL3 basic EGFP	EGFP reporter vector with restriction sites to clone in a promoter of interest, derived from pGL3 basic
128054	pGL3 promoter EGFP	EGFP reporter vector with restriction sites to clone in an enhancer of interest, derived from pGL3 promoter
128055	plenti guide ds red	Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.
128057	pcdna3.1 hygro mCherry	Mammalian expression vector with N terminal mCherry
128058	pFastbac1 Nterminal GST	Baculovirus expression vector with N terminal GST
128059	pCDH-EF1-IRES eGFP	Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
128060	pScarlet-N1	Mammalian expression vector for C terminal mScarlet-I fusions
128061	pLVX-Tet3G blasticidin	Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.
128062	pLenti6-V5-Topo mCherry	Lentiviral vector for C terminal mCherry fusions
128063	plenti guide Thy1.1	Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.
128064	BS II KS+ loxP-GFP_ZEO_loxP	has loxp-GFP-Zeo-Loxp cassette
128065	pShuttle-CMV-GFP	Adenoviral shuttle vector with CMV promoter-EGFP
128067	FUGW ds red	Lentiviral expression of dsRed Express2
128068	FUGW ECFP	Lentiviral expression of ECFP
128069	pREVtre GFP	Retroviral tet inducible EGFP
128070	pLVX TRE 3G BFP	Lentiviral expression of Tet inducible TagBFP
128071	FUGW mplum	3rd gen lentiviral plasmid with hUbC-driven mPlum
128072	pCDH-EF1-IRES plum	gfp was exchanged to plum
128073	pLKO.1 mCherry	puro exchanged to mCherry
128074	FUGW-EYFP	3rd gen lentiviral plasmid with hUbC-driven EYFP
129427	Lenti puro EF2 LUC-2A-Dendra2	expression of LUC and Dendra2 reporters under EF2 promoter
129428	pLOC CMV-IRES-Dsred-blasticidin	expression of Dsred-blasticidin under CMVpromoter -IRES
129429	plex.mcs sv40promoter-luc	Luc expression under SV40 promoter
129431	EF1a mCherry	expression of mCherry from EF1a promoter
129432	EF1a CHR2 mCherry WPRE	expression of CHR2 mCherry from EF1a promoter
129433	pRS426 TEF mCherry	express mcherry
129435	pTRE2 EGFP	express GFP
129436	pCDH-UBC-LUC-EF1 Hygro	express LUC
129437	pCDH-UBC-DSRED-LUC-EF1 Hygro	express dsred-luc chimera
129438	pCDH-UBC-irfp luc-EF1 Hygro	express irfp-luc chimera
129439	pCDH-UBC-dendra 2LUC-EF1 Hygro	express dendra2-luc chimera
129440	pCDH-CMV-mCherry-EF1 Hygro	express mCherry
129441	pGEX-4T1 NLS-GFP	bacterial expression of GFP
129442	pTXB1 EGFP	expression of intein/ gfp
129443	EF1a- gfp-2a-puro	expression of GFP and Puro R
129444	pCDNA3.3 CAS9 IRES-Dsred	expression of FnCas9 and DsRed
129445	pET28a HAT epitope	expression of HAT epitope