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Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing.
Tapscott T, Guarnieri MT, Henard CA
Appl Environ Microbiol. 2019 May 16;85(11). pii: AEM.00340-19. doi: 10.1128/AEM.00340-19. Print 2019 Jun 1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
128159pCAH01SpRpCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS
128160pCAH01Sp::Cas9Ptet-controlled expression of S. pyogenes Cas9
128161pCAH01Sp::Cas9D10APtet-controlled expression of S. pyogenes Cas9D10A variant
164160pQCHIncQ BHR plasmid