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(Plasmid #128160)


Item Catalog # Description Quantity Price (USD)
Plasmid 128160 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 7755
  • Total vector size (bp) 11704
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Sp Cas9
  • Species
    S. pyogenes
  • Insert Size (bp)
  • Promoter Ptet

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCC GAC ACC ATC GAA TGG CCA GAT G
  • 3′ sequencing primer CAG GGC GCG TGG AGA TCC GT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAH01Sp::Cas9 was a gift from Calvin Henard (Addgene plasmid # 128160 ; ; RRID:Addgene_128160)
  • For your References section:

    Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing. Tapscott T, Guarnieri MT, Henard CA. Appl Environ Microbiol. 2019 May 16;85(11). pii: AEM.00340-19. doi: 10.1128/AEM.00340-19. Print 2019 Jun 1. 10.1128/AEM.00340-19 PubMed 30926729