pCAH01
(Plasmid #128162)

• Purpose: (Empty Backbone) IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria
• Depositing Labs: Michael Guarnieri, Calvin Henard
• Publication: Henard et al Sci Rep. 2016 Feb 23;6:21585. doi: 10.1038/srep21585.

Backbone

• Vector backbone: pAWP78
• Backbone size (bp): 6400
• Modifications to backbone: A ~2400bp fragment containing the inducible tetracycline promoter/operator and tetR from pASK75 was cloned via Gibson Assembly to generate a broad host range inducible expression vector
• Vector type: Bacterial Expression
• Promoter: Ptet
• Selectable markers: ampicillin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCC GAC ACC ATC GAA TGG CCA GAT G
• 3′ sequencing primer: CAG GGC GCG TGG AGA TCC GT

Resource Information

• Supplemental Documents:
  • pCAH01.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pAWP78 was gifted from Mary Lidstrom's laboratory at the University of Washington