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The MEMIC is an ex vivo system to model the complexity of the tumor microenvironment.
Janska L, Anandi L, Kirchberger NC, Marinkovic ZS, Schachtner LT, Guzelsoy G, Carmona-Fontaine C
Dis Model Mech. 2021 Aug 1;14(8). pii: 271783. doi: 10.1242/dmm.048942. Epub 2021 Aug 18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
128958pLenti-5HRE-GFP PuroRLentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein
128960pLenti-5HRE-GFP NeoRLentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein
129285pLenti-mCherry-CAAXLentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence
166228pLenti-mCherry-CAAX PuroRLentiviral plasmid with Puromycin selection for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane
166229pLenti-mCherry-CAAXLentiviral plasmid for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane.

Antibodies from Article