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Silvestro Conticello Lab: New frontiers to cure Alport syndrome: COL4A3 and COL4A5 gene editing in podocyte-lineage cells
Unpublished

Plasmids from Article

ID Plasmid Purpose  
130279pAAV_Donor/Reporter-COL4A3COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template
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130280pAAV_Donor/Reporter-COL4A5COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template
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130281pAAV_COL4A3_self-cleavingCas9Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation
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130282pAAV_COL4A5_self-cleavingCas9Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation
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