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Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, Nureki O, Tomita M, Aburatani H, Yachie N
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
131124pRS0045Mammalian Target-ACE expression vector
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131125pRS0035Mammalian Target-AID expression vector
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131126pLV-SI-121A-to-G base editing reporter
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131127pLV-SI-112C-to-T base editing reporter
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131130pSI-535gRNA expression vector for C-to-T base editing reporter
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131131pSI-359gRNA expression vector for A-to-G base editing reporter
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139105pCMV-Target-ACEmaxMammalian Target-ACEmax expression vector (pSI-625)
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139106pCMV-ACBEmaxMammalian ACBEmax expression vector (pSI-632)
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139107pCMV-Target-AIDmaxMammalian Target-AIDmax expression vector (pSI-626)
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Antibodies from Article