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Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, Nureki O, Tomita M, Aburatani H, Yachie N
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
131124pRS0045Mammalian Target-ACE expression vector
131125pRS0035Mammalian Target-AID expression vector
131126pLV-SI-121A-to-G base editing reporter
131127pLV-SI-112C-to-T base editing reporter
131130pSI-535gRNA expression vector for C-to-T base editing reporter
131131pSI-359gRNA expression vector for A-to-G base editing reporter
139105pCMV-Target-ACEmaxMammalian Target-ACEmax expression vector (pSI-625)
139106pCMV-ACBEmaxMammalian ACBEmax expression vector (pSI-632)
139107pCMV-Target-AIDmaxMammalian Target-AIDmax expression vector (pSI-626)

Antibodies from Article