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Assessment of Cas12a-mediated gene editing efficiency in plants.
Bernabe-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, Garcia-Carpintero V, Gianoglio S, Vazquez-Vilar M, Granell A, Orzaez D
Plant Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
160572pUPD2 AsCas12a (GB1438)Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).
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160573pUPD2 LbCas12a (GB1439)Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).
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160574pUPD2 AtU6-26:AsCas12a_DR (GB1442)AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.
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160575pUPD2 AtU6-26:LbCas12a_DR (GB1443)LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.
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160576pUPD2 HDV (GB1444)Hepatitis Delta Virus ribozyme signal for the assembly of gRNA expression cassettes.
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160594pUPD2 AtUBQ10 (GB2478)UBQ10 promoter from A. thaliana for constitutive expression in plant systems.
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160610pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.
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160611pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.
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Antibodies from Article