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Antimicrobial solid media for screening non-sterile Arabidopsis thaliana seeds.
Behrendorff JBYH, Borras-Gas G, Pribil M
Physiol Plant. 2020 Feb 25. doi: 10.1111/ppl.13079.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
132565pN_35S/mEGFPmEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)
132566pN_35S/mApplemApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)
132568GS2.1/ECsgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).
135320pB_35S/mEGFPmEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)
135321pH_35S/mEGFPmEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

Antibodies from Article