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pN_35S/mEGFP
(Plasmid #132565)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 132565 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pN_35S
  • Backbone size w/o insert (bp) 9267
  • Total vector size (bp) 9987
  • Vector type
    Plant Expression
  • Selectable markers
    Nourseothricin/streptothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mEGFP
  • Alt name
    monomeric enhanceed green fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter Cauliflower mosaic virus 35S

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTCGGATTCCATTGCCCAGCTAT
  • 3′ sequencing primer ATATGCTCAACACATGAGCGA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mEGFP was amplified from mEGFP-pBAD, a gift from Michael Davidson (Addgene plasmid # 54622 ; http://n2t.net/addgene:54622 ; RRID:Addgene_54622).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/852020v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN_35S/mEGFP was a gift from Mathias Pribil (Addgene plasmid # 132565 ; http://n2t.net/addgene:132565 ; RRID:Addgene_132565)
  • For your References section:

    Antimicrobial solid media for screening non-sterile Arabidopsis thaliana seeds. Behrendorff JBYH, Borras-Gas G, Pribil M. Physiol Plant. 2020 Feb 25. doi: 10.1111/ppl.13079. 10.1111/ppl.13079 PubMed 32096870