A method for cost-effective and rapid characterization of engineered T7-Based transcription factors by cell-free protein synthesis reveals insights into the regulation of T7 Rna polymerase-driven expression.
McManus JB, Lux MW, Murray RM, Emanuel PA
Arch Biochem Biophys. 2019 Jul 18. pii: S0003-9861(19)30288-7. doi: 10.1016/j.abb.2019.07.010. PubMed Article
McManus JB, Lux MW, Murray RM, Emanuel PA
Arch Biochem Biophys. 2019 Jul 18. pii: S0003-9861(19)30288-7. doi: 10.1016/j.abb.2019.07.010. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133521 | pY71-tet.I.8 | sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site |
| 133523 | pY71-tet.I.1 | sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site |
| 133524 | pY71-tet.I.2 | sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site |
| 133525 | pY71-tet.I.3 | sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site |
| 133526 | pY71-tet.I.4 | sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site |
| 133527 | pY71-tet.I.5 | sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site |
| 133528 | pY71-tet.I.6 | sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site |
| 133529 | pY71-tet.I.7 | sfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start site |
| 133530 | pY71-tet.I.9 | sfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start site |
| 133531 | pY71-tet.I.10 | sfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start site |
| 133532 | pY71-tet.I.11 | sfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site |
| 133533 | pY71-tet.I.12 | sfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start site |
| 133534 | pY71-tet.I.13 | sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site |
| 133535 | pY71-tet.I.14 | sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site |
| 133536 | pY71-tet.I.16 | sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site |
| 133537 | pY71-tet.I.20 | sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site |
| 133538 | pY71-tet.I.25 | sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site |
| 133539 | pY71-tet.I.-1 | sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site |