pY71-tet.I.1
(Plasmid #133523)

• Purpose: sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site
• Depositing Lab: Richard Murray
• Publication: McManus et al Arch Biochem Biophys. 2019 Jul 18. pii: S0003-9861(19)30288-7. doi: 10.1016/j.abb.2019.07.010.

Backbone

• Vector backbone: PY71
• Backbone size w/o insert (bp): 1549
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sfGFP
• Alt name: T7 promoter
• Alt name: tetO
• Species: Synthetic
• Insert Size (bp): 1071
• Mutation: WT
• Promoter: T7
• Tags / Fusion Proteins:
  • TEV site (N terminal on backbone)
  • Strep tag (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TTGTGATGCTCGTCAGGG
• 3′ sequencing primer: CTGCCTCGGTGAGTTTTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry