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High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803.
Dienst D, Wichmann J, Mantovani O, Rodrigues JS, Lindberg P
Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.1038/s41598-020-62681-w. PubMed Article

Plasmids from Article

ID Plasmid Purpose
133970pSHDY*in_PpetE:BCD2:mVenusHeterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.
133971pSHDY*in_PpetE:BCD2:AgBIS:FlagHeterologous, copper-inducible expression of (E)-α-bisabolene synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. The AgBIS CDS is fused to a C-termial Flag-tag.
133973pSHDY*in_PpetE:BCD2:MrBBS:StrepHeterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.

Antibodies from Article