A Targetron-Recombinase System for Large-Scale Genome Engineering of Clostridia.
Cerisy T, Rostain W, Chhun A, Boutard M, Salanoubat M, Tolonen AC
mSphere. 2019 Dec 11;4(6). pii: 4/6/e00710-19. doi: 10.1128/mSphere.00710-19. PubMed Article
Cerisy T, Rostain W, Chhun A, Boutard M, Salanoubat M, Tolonen AC
mSphere. 2019 Dec 11;4(6). pii: 4/6/e00710-19. doi: 10.1128/mSphere.00710-19. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135655 | pQlox71F | Clostridial vector, encoding a targetron carrying a lox71 site in the forward (sense) orientation. Retarget intron to insert lox71 onto genome at desired site. |
| 135656 | pQlox71R | Clostridial vector, encoding a targetron carrying a lox71 site in the reverse (antisense) orientation. Retarget intron to insert lox71 onto genome at desired site. |
| 135657 | pQlox66R | Clostridial vector, encoding a targetron carrying a lox66 site in the reverse (antisense) orientation. Retarget intron to insert lox66 onto genome at desired site. |
| 135658 | pQlox66F | Clostridial vector, encoding a targetron carrying a lox66 site in the forward (sense) orientation. Retarget intron to insert lox66 onto genome at desired site. |
| 135659 | pQcre1 | Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites. |
| 135660 | pQadd1F | Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site. |
| 135661 | pQadd1R | Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location. |
| 135662 | pQadd2 | Clostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R. |