PurposeClostridial vector, encoding the Cre protein, which mediates recombination between lox sites.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||135659||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6500
- Total vector size (bp) 7752
Vector typeBacterial Expression
Growth in Bacteria
Growth instructions200 µg/mL erythromycin
Copy numberHigh Copy
Gene/Insert nameCre recombinase
Insert Size (bp)1032
- Promoter ppag promoter
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CTTCCGGCTCGTATGTTGTG
- 3′ sequencing primer TACATCACCGACGAGCAAGG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byCre expression cassette cloned from pRAB1 provided by Dr. Ralph Bertram and Christopher F. Schuster
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cre protein under the control of the gram + PpagA promoter.
Leibig M, Krismer B, Kolb M, Friede A, Götz F, Bertram R. 2008. Marker removal in staphylococci via Cre recombinase and different lox sites. Appl Environ Microbiol 74:1316– 1323.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQcre1 was a gift from Andrew Tolonen (Addgene plasmid # 135659 ; http://n2t.net/addgene:135659 ; RRID:Addgene_135659)
For your References section:A Targetron-Recombinase System for Large-Scale Genome Engineering of Clostridia. Cerisy T, Rostain W, Chhun A, Boutard M, Salanoubat M, Tolonen AC. mSphere. 2019 Dec 11;4(6). pii: 4/6/e00710-19. doi: 10.1128/mSphere.00710-19. 10.1128/mSphere.00710-19 PubMed 31826971