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An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells.
Xie Y, Wang D, Lan F, Wei G, Ni T, Chai R, Liu D, Hu S, Li M, Li D, Wang H, Wang Y
Sci Rep. 2017 May 24;7(1):2320. doi: 10.1038/s41598-017-02456-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
135960epiCRISPRExpresses SpCas9, and cloning backbone for sgRNA
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135961epiCRISPRnExpresses SpCas9, and cloning backbone for sgRNA
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135962epiCRISPReExpresses SpCas9, and cloning backbone for sgRNA
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135963gRNAU6Contains tracrRNA-hU6 sequence for double sgRNA cloning
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Recombinant Antibodies from Article