Purpose(Empty Backbone) Expresses SpCas9, and cloning backbone for sgRNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||135961||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneepiCRISPR plasmid
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:epiCRISPRn was a gift from Yongming Wang (Addgene plasmid # 135961 ; http://n2t.net/addgene:135961 ; RRID:Addgene_135961)
For your References section:An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells. Xie Y, Wang D, Lan F, Wei G, Ni T, Chai R, Liu D, Hu S, Li M, Li D, Wang H, Wang Y. Sci Rep. 2017 May 24;7(1):2320. doi: 10.1038/s41598-017-02456-y. 10.1038/s41598-017-02456-y PubMed 28539611