DiCre-Based Inducible Disruption of Leishmania Genes.
Duncan SM, Myburgh E, Alves-Ferreira EV, Mottram JC
Methods Mol Biol. 2019;1971:211-224. doi: 10.1007/978-1-4939-9210-2_10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137047 | pGL2206 | Drug Resistance pDONR221SAT |
| 137048 | pGL2207 | Drug Resistance pDONR221HYG |
| 137049 | pGL2208 | Drug Resistance pDONR221BSD |
| 137050 | pGL2209 | Drug Resistance pDONR221 PUR |
| 137051 | pGL2399 | Expression of rapamycin-inducible dimerisation of split Cre from ribosomal locus in Leishmania parasite; pRIB-DiCre |
| 137053 | pGL2313 | Endogenous Locus DiCre Expression Cassette |
| 137054 | pGL2314 | loxP (empty) expression cassette: c-terminal 6xHA |
| 137055 | pGL2315 | loxP (empty) expression cassette: c-terminal GFP |
| 137063 | pGL2316 | loxP (empty) expression cassette: n-terminal GFP |