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Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Levy JM, Yeh WH, Pendse N, Davis JR, Hennessey E, Butcher R, Koblan LW, Comander J, Liu Q, Liu DR
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
137171CMV_Npu-BE3.9max N-terminalExpresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter
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137172CMV_Npu-BE3.9max C-terminalExpresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter
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137173CMV_Npu-ABEmax N-terminalExpresses the N-terminal split-intein fragment of ABEmax from the CMV promoter
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137174CMV_Npu-ABEmax C-terminalExpresses the C-terminal split-intein fragment of ABEmax from the CMV promoter
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137175Cbh_v5 AAV-CBE N-terminalAAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA
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137176Cbh_v5 AAV-CBE C-terminalAAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA
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137177Cbh_v5 AAV-ABE N-terminalAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter
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137178Cbh_v5 AAV-ABE C-terminalAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA
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137179CMV_Npu-saBE3.9max N-terminalExpresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter
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137180CMV_Npu-saBE3.9max C-terminalExpresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter
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137181CMV_Npu-saBE3.9max_KKH C-terminalExpresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter
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137182Cbh_v5 AAV-saCBE N-terminalAAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNA
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137183Cbh_v5 AAV-saCBE C-terminalAAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, and U6-sgRNA
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137184Cbh_v5 AAV-saCBE_KKH C-terminalAAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE, and U6-sgRNA (KKH variant).
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