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Cbh_v5 AAV-saCBE N-terminal
(Plasmid #137182)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 137182 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    v5 AAV-saCBE N-terminal
  • Alt name
    JL842
  • gRNA/shRNA sequence
    BsmBI dummy protospacer
  • Species
    Synthetic
  • Mutation
    Cas9 D10A
  • Promoter Cbh

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Depositor Comments

SaCas9 split between Lys534/Cys535; clone in protospacer by cutting with BsmBI/Esp3I and ligating annealed oligos as described in methods. Inserts were Gibson-cloned into BshTI/BglII-restricted backbones to avoid ITR PCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cbh_v5 AAV-saCBE N-terminal was a gift from David Liu (Addgene plasmid # 137182 ; http://n2t.net/addgene:137182 ; RRID:Addgene_137182)
  • For your References section:

    Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Levy JM, Yeh WH, Pendse N, Davis JR, Hennessey E, Butcher R, Koblan LW, Comander J, Liu Q, Liu DR. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. 10.1038/s41551-019-0501-5 PubMed 31937940