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Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.
Stefely JA, Zhang Y, Freiberger EC, Kwiecien NW, Thomas HE, Davis AM, Lowry ND, Vincent CE, Shishkova E, Clark NA, Medvedovic M, Coon JJ, Pagliarini DJ, Mercer CA
Autophagy. 2020 Jan 23. doi: 10.1080/15548627.2020.1719746.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
137754GST-RFP-cb5Targets red flourescent protein (RFP) to the endoplasmic reticulum
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137755GST-Keima-cb5Targets Keima to the endoplasmic reticulum. Used to monitor ER-phagy
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137756HA-MAP1LC3AExpression of LC3A
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137757HA-MAP1LC3BExpression of LC3B
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137758HA-MAP1LC3CExpression of LC3C
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137759HA-GABARAPL1Expression of GABARAPL1
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137760HA-GABARAPExpression of GABARAP
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137761HA-GABARAPL2Expression of GABARAPL2
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137762NoTag MAP1LC3CExpression of LC3C without epitope tags
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137763HA-GFPControl plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.
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137764GFP-DDKControl plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.
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137765GFP-CALCOCO1Expression and localization of tagged CALCOCO1
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137766CALCOCO1-HAExpression and localization of tagged CALCOCO1
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137767CALCOCO1 L140A-mycDDKCALCOCO1 CLIR mutant
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137768CALCOCO1 V142A-mycDDKCALCOCO1 CLIR mutant
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137769CALCOCO1 W47A-mycDDKCALCOCO1 LIR mutant
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137770CALCOCO1 R12H-mycDDKCALCOCO1 R12H mutant
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Antibodies from Article