PurposeExpression of LC3B
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||137757||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneMAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
- Promoter CMV
/ Fusion Proteins
- HA (N terminal on insert)
- myc (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
3' codons have been altered from original reference sequence, while preserving the amino acid sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HA-MAP1LC3B was a gift from Carol Mercer (Addgene plasmid # 137757 ; http://n2t.net/addgene:137757 ; RRID:Addgene_137757)
For your References section:Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. Stefely JA, Zhang Y, Freiberger EC, Kwiecien NW, Thomas HE, Davis AM, Lowry ND, Vincent CE, Shishkova E, Clark NA, Medvedovic M, Coon JJ, Pagliarini DJ, Mercer CA. Autophagy. 2020 Jan 23. doi: 10.1080/15548627.2020.1719746. 10.1080/15548627.2020.1719746 PubMed 31971854