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Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917.
Kan A, Gelfat I, Emani S, Praveschotinunt P, Joshi NS
ACS Synth Biol. 2020 Dec 10. doi: 10.1021/acssynbio.0c00466. PubMed Article

Plasmids from Article

ID Plasmid Purpose
137921pM1s3AsGE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression
137922pM1s3TsRE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expression
137923pM2s2TsRE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression
137924pM2s2AsGE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression
137944pM2s2AsR_TS-E10E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
137945pM1s3AsR_TS-C7E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
137946pM1s3ATScsg-EtagE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expression
141293pCryptDel4.8Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.

Antibodies from Article