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An expanded library of orthogonal split inteins enables modular multi-peptide assemblies.
Pinto F, Thornton EL, Wang B
Nat Commun. 2020 Mar 23;11(1):1529. doi: 10.1038/s41467-020-15272-2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
138167pFP.R265Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)
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138175pFP.R400Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)
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138179pFP.R267Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)
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138180pFP.R268Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)
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138181pFP.R269Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)
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138219pFP.R270Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)
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138220pFP.R271Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)
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138221pFP.R272Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)
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138222pFP.R401Expresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)
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138223pFP.R274Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)
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138224pFP.R275Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)
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138225pFP.R355Expresses the split mCherry reporter interrupted by the Cro-RIR1 artificially mini-intein (in cis)
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138226pFP.R356Expresses the split mCherry reporter interrupted by the Cro-RPB2 intein (in cis)
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138227pFP.R357Expresses the split mCherry reporter interrupted by the Cro-Top2 artificially mini-intein (in cis)
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138228pFP.R358Expresses the split mCherry reporter interrupted by the CIV-RIR1 artificially mini-intein (in cis)
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138229pFP.R359Expresses the split mCherry reporter interrupted by the CP-Thy1 artificially mini-intein (in cis)
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138230pFP.R360Expresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)
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138231pFP.R361Expresses the split mCherry reporter interrupted by the BsuP-RIR1 artificially mini-intein (in cis)
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138232pFP.R362Expresses the split mCherry reporter interrupted by the CbP-RNR artificially mini-intein (in cis)
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138233pFP.R363Expresses the split mCherry reporter interrupted by the Ckl-Ter artificially mini-intein (in cis)
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138234pFP.R364Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)
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138235pFP.R365Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)
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138236pFP.R366Expresses the split mCherry reporter interrupted by the LLP-Pol intein (in cis)
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138237pFP.R367Expresses the split mCherry reporter interrupted by the LP-Hel intein (in cis)
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138238pFP.R368Expresses the split mCherry reporter interrupted by the MP-B-DnaB artificially mini-intein (in cis)
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138239pFP.R369Expresses the split mCherry reporter interrupted by the MP-K-gp53 intein (in cis)
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138240pFP.R370Expresses the split mCherry reporter interrupted by the MP-C-gp206 artificially mini-intein (in cis)
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138241pFP.R371Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)
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138242pFP.R372Expresses the split mCherry reporter interrupted by the PP-Phi artificially mini-intein (in cis)
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138243pFP.R373Expresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)
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138244pFP.R374Expresses the split mCherry reporter interrupted by the SaP-Hel artificially mini-intein (in cis)
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138245pFP.R375Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)
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138246pFP.R376Expresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)
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138247pFP.R377Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)
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138248pFP.E222Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters
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138249pFP.E227Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters
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Recombinant Antibodies from Article